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1.
Mem Inst Oswaldo Cruz ; 115: e190338, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32130368

RESUMO

Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses infecting humans in Brazil, primarily in the Amazon Region. Here, we report the detection of OROV in the saliva and urine of a patient whose samples were collected five days after the onset of symptoms. Nucleotide sequencing and phylogenetic analysis further confirmed the results. To our knowledge, this is the first study reporting the detection of OROV in the saliva and urine of an infected patient. In addition, the results of our study expand the current knowledge pertaining to the natural history of Oropouche fever.


Assuntos
Infecções por Bunyaviridae/diagnóstico , Orthobunyavirus/genética , Orthobunyavirus/isolamento & purificação , Saliva/virologia , Urina/virologia , Sequência de Aminoácidos , Sequência de Bases , Feminino , Humanos , Pessoa de Meia-Idade , Filogenia , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
2.
Mem. Inst. Oswaldo Cruz ; 115: e190338, 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1091240

RESUMO

Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses infecting humans in Brazil, primarily in the Amazon Region. Here, we report the detection of OROV in the saliva and urine of a patient whose samples were collected five days after the onset of symptoms. Nucleotide sequencing and phylogenetic analysis further confirmed the results. To our knowledge, this is the first study reporting the detection of OROV in the saliva and urine of an infected patient. In addition, the results of our study expand the current knowledge pertaining to the natural history of Oropouche fever.


Assuntos
Humanos , Feminino , Saliva/virologia , Urina/virologia , Orthobunyavirus/isolamento & purificação , Orthobunyavirus/genética , Infecções por Bunyaviridae/diagnóstico , Filogenia , RNA Viral/genética , Sequência de Bases , Sequência de Aminoácidos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Pessoa de Meia-Idade
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